In vivo near-infrared fluorescence imaging of F8-IL10 fusion proteins in mice with collagen-induced arthritis. Arthritic mice (n = 1) were injected intravenously with 200 or 100 μg F8-muIL10 or F8-huIL10 labeled with IRDye 750. (a) Mice injected with 200 μg of fusion protein were imaged 1, 4, 24 and 48 hours after injection. (b) After 48 hours mice were sacrificed and individual paws were imaged. In addition, photographs of paws are shown to illustrate the paw swelling. (c) Mice injected with 100 μg fusion protein were imaged 1, 4, and 24 hours after injection. (d) After 24 hours mice were sacrificed and individual paws were imaged. Indicated numbers represent the score of the according paw: 1, one toe inflamed and swollen; 2, more than one toe, but not entire paw, inflamed and swollen or mild swelling of entire paw; 3, entire paw inflamed and swollen. FL, front left; FR, front right; BL, back left; BR, back right; plus the according score. *Site of immunization.
Doll et al. Arthritis Research & Therapy 2013 15:R138 doi:10.1186/ar4319