Table 3

RT-PCR primers sequences (1st BASE Pte. Ltd., Singapore)
Gene RT-PCR primer sequences Tm (°C) GC (%)
Collagen II (Forward) 5′-CTG GAT GCC ATG AAG GTT TT-3′ 58.4 45.0
Collagen II (Reverse) 5′-TCT TGT CCT TGC TCT TGC TG-3′ 60.4 50.0
Aggrecan (Forward) 5′-GCA AGT GGT CTT CCT TCT GG-3′ 62.4 55.0
Aggrecan (Reverse) 5′-TTC CAC CAA TGT CGT ATC CA-3′ 58.4 45.0
Sox 9 (Forward) 5′-TGA AGA AGG AGA GCG AGG AG-3′ 62.4 55.0
Sox 9 (Reverse) 5′-GAC GTG CGG CTT GTT CTT-3′ 59.9 55.6
GAPDH (Forward) 5′-GCT CTC TTC CAG CCT TCC TT-3′ 62.4 55.0
GAPDH (Reverse) 5′-TAG AGG TCC TTG CGG ATG TC-3′ 62.4 55.0

GC (%), GC content; Tm (°C), Melting temperature.

Nam et al.

Nam et al. Arthritis Research & Therapy 2013 15:R129   doi:10.1186/ar4309

Open Data