Additional file 6: Figure S5.

Showing that primary cultured chondrocytes were subcultured twice and then cultured in monolayers in the presence or absence of echistatin (1 μM). Seven days later, RNA was obtained, complementary DNA was synthesized, and expression of type II procollagen (COL2A1), aggrecan (ACAN), type I procollagen (COL1A1) and type III procollagen (COL3A1) were evaluated by quantitative PCR. Results are shown by relative ratios against the cells cultured without echistatin (open bars). Results are mean ± standard error of the mean of three independent experiments, each in triplicate. **P <0.01 against cells cultured without echistatin. GADPH, glyceraldehyde 3-phosphate dehydrogenase.

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Tanaka et al. Arthritis Research & Therapy 2013 15:R127   doi:10.1186/ar4307