Open Access Research article

Dynamic pressurization induces transition of notochordal cells to a mature phenotype while retaining production of important patterning ligands from development

Devina Purmessur1, Clare C Guterl1, Samuel K Cho1, Marisa C Cornejo1, Ying W Lam2, Bryan A Ballif2, Damien M Laudier1 and James C Iatridis1*

Author Affiliations

1 Leni and Peter W. May Department of Orthopedics, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA

2 Department of Biology, University of Vermont, Burlington, VT 05405, USA

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Arthritis Research & Therapy 2013, 15:R122  doi:10.1186/ar4302

Published: 17 September 2013

Additional files

Additional file 1:

Table S1 presenting the full table of proteomics data for intracellular protein. All peptides identified related to intracellular proteins in NCCM from the Control and daily pressurization groups. Proteins identified by mass spectrometry from the Control and Daily load samples. These two samples were chosen as we anticipate that these two groups would yield the greatest magnitude difference in effects. Proteins were identified as described in Methods. Protein symbols including hyperlinks are provided. Proteins are categorized into either secreted/extracellular or primarily intracellular proteins. Three Control and Daily Load samples are shown with the total number of peptides identified from each indicated protein. Also provided are the average number of peptides identified from Control or Daily Load samples, the standard deviation (STD), the difference (DL-C) and results of a Student's t test comparing the two means.

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Additional file 2:

Table S2 presenting the full table of proteomics data for extracellular proteins. All peptides identified related to extracellular proteins in NCCM from the Control and daily pressurization groups. Proteins were identified using the same method as for intracellular proteins.

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Open Data