Table 1

Values of mitochondrial respiratory chain complexes in cultures of normal chondrocytes treated with two NO donor compounds

Normal

chondrocytes

Cells treated with

1 mM NOC-12

Cells treated with

1 mM SNP


Age, years

49.8 ± 21.2 (14)

44.2 ± 19.4 (5)

59.7 ± 18.9 (11)

Proteins, mg/ml

3.1 ± 0.9 (14)

2.8 ± 1.4 (5)

4.2 ± 1.4 (11)

CS enzymatic activity (nmol/min/mg protein)

108.9 ± 38.5 (14)

146.9 ± 54.7 (5)

106.6 ± 26.2 (11)

Mitochondrial complex activitya

Complex I

30.1 ± 13.4 (14)

6.8 ± 2.3 (5)*

22.8 ± 19.1 (11)

Complex II

7.4 ± 2.3 (14)

6.8 ± 1.6 (5)

10.2 ± 1.81 (11)

Complex III

53.5 ± 18.4 (14)

35.2 ± 9.3 (5)*

46.3 ± 9.7 (11)

Complex IV

61.2 ± 8.3 (14)

15.7 ± 3.1 (5)*

40.2 ± 11.3 (11)*


Values are the mean ± SD (number). *P ≤ 0.05 versus untreated chondrocytes. aCS-corrected complex activity is expressed as (nmol/minute/mg protein)/(CS-specific activity) × 100. NO, nitric oxide; NOC-12, N-ethyl-2(1-ethyl-2 hydroxy-2-nitrosohydrazine; CS, citrate synthase; Complex I, rotenone-sensitive NADH-coenzime Q1 reductase; complex II, succinate dehydrogenase; complex III, antimycin-sensitive ubiquinol cytochrome c reductase; complex IV, cytochrome c oxidase. Chondrocytes were treated with NOC-12 for 24 hours and SNP for 5 hours.

de Andrés et al. Arthritis Research & Therapy 2013 15:R115   doi:10.1186/ar4295

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