IL-2 and IL-8 are produced in larger quantities in suppression experiments with effector cells primed by DexVD3 DC. The supernatants were collected before the FACS analysis of proliferation of the responder cells and stored in aliquots at -20°C until analysis. The amount of cytokines/chemokines secreted were analyzed using a 25-plex bead assay and the concentrations are given in pg/ml. Cytokines and chemokines that showed results below the detection limit (IL-1β, IL-4, Eotaxin, GM-CSF, IL-17), and those that where the differences were not significant, are not shown. The bars indicate the median, n = 4. The differences between the secreted amounts of cytokines are marked with the following: *P ≤ 0.05.
Volchenkov et al. Arthritis Research & Therapy 2013 15:R114 doi:10.1186/ar4294