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Open Access Research article

In vitro suppression of immune responses using monocyte-derived tolerogenic dendritic cells from patients with primary Sjögren's syndrome

Roman Volchenkov1, Johan G Brun23, Roland Jonsson12 and Silke Appel1*

Author Affiliations

1 Broegelmann Research Laboratory, Department of Clinical Science, University of Bergen, Jonas Lies vei 65, 5021 Bergen, Norway

2 Department of Rheumatology, Haukeland University Hospital, Jonas Lies vei 65, 5021 Bergen, Norway

3 Section for Rheumatology, Department of Clinical Science, University of Bergen, Jonas Lies vei 65, 5021 Bergen, Norway

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Arthritis Research & Therapy 2013, 15:R114  doi:10.1186/ar4294

Published: 9 September 2013

Abstract

Introduction

Therapeutic vaccination with antigen-specific tolerogenic dendritic cells (tolDC) might become a future option of individualized therapy for patients with autoimmune diseases. In this study, we tested the possibility of generating monocyte-derived tolDC from patients with primary Sjögren's syndrome (pSS). We analyzed phenotype, cytokine production and ability to suppress Ro/La-specific immune responses.

Methods

Monocyte-derived tolDC from patients with pSS were generated in the presence of dexamethasone, vitamin D3 and lipopolysaccharide (DexVD3 DC). The phenotype was analyzed by flow cytometry and the cytokine profile was investigated using a 25-plex Luminex assay and ELISA. The capacity to both stimulate Ro/La-specific T cells and suppress this response was evaluated by autologous mixed lymphocyte reaction (MLR).

Results

DC generated from patients with pSS had a similar phenotype and cytokine profile to those from healthy controls. DexVD3 DC from pSS patients induced little antigen-specific T cell proliferation, but DexVD3 DC-primed lymphocytes successfully suppressed Ro/La-specific T cell responses.

Conclusions

DexVD3 DC presenting Ro/La antigens might be a promising new therapeutic option for patients with pSS.

Keywords:
Sjögren's syndrome; dendritic cells; immunotherapy; Ro/La-specific T cell suppression