CD148 mRNA in mouse collagen-induced arthritis model. RNA was extracted from whole rear paws from the CIA mouse model, reverse transcribed and used to assess the expression of CD148 by quantitative real-time PCR. CIA score 0 refers to normal joints and 3 to severely inflamed joints marked by rigidity or joint distortion. The axis shows the relative expression of ptprj (CD148) compared to hprt, a control housekeeping gene. Data points (+/- SD) represent the average of triplicate samples each from triplicate independent experimental tissues, n = 3. CIA, collagen-induced arthritis; PCR, polymerase chain reaction.
Dave et al. Arthritis Research & Therapy 2013 15:R108 doi:10.1186/ar4288