miR-31 is one of the highly upregulated microRNAs during osteoclastogenesis. (A) The profile of microRNA (miRNA) expression levels was visualized by a scatter plot of signal intensities. Horizontal axis, cells treated with macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor κB ligand (RANKL) for 24 hours (BM-Oc); vertical axis, cells treated only with M-CSF (bone marrow macrophage (BMM)). Dashed red lines indicate 1.5-fold upregulated or downregulated point, and miR-31 is indicated as a red dot. (B) Expression of miR-31 during osteoclastogenesis was examined by real-time quantitative PCR at the indicated time points. Values were normalized against U6B. RANKL treatment of BMMs (BM-Oc) enhanced the expression of miR-31. (C) Real-time quantitative PCR analysis of miR-31 after the transduction with retroviruses encoding NFATc1 (pMX-NFATc1) or control viruses (pMX-control). Overexpression of NFATc1 did not increase the level of miR-31. Nfatc1, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1.
Mizoguchi et al. Arthritis Research & Therapy 2013 15:R102 doi:10.1186/ar4282