Table 7

Real-time PCR analysis of 14 selected genes using a different set of donors reveals similar correlation with RNA-Seq results

Gene name

Differential expression

Age

P value


Young

Old


DKK1

Higher in old

0.0004 ± 0.0006

0.008 ± 0.0006

0.04

COL10

Higher in old

7.66 × 10-5 ± 3.05 × 10-5

0.000148 ± 7.13 × 10-5

0.1

RUNX2

Lower in old

0.002 ± 0.0004

0.0005 ± 0.0004

0.02

SRPX

Lower in old

0.0025 ± 0.0002

0.001 ± 0.002

0.05

ACSL5

Lower in old

0.004 ± 0.002

0.002 ± 0.02

0.13

IL7R

Lower in old

0.001 ± 0.0002

0.0007 ± 0.0004

0.07

COL2A1

Lower in old

32.6 ± 24.5

0.2 ± 0.13

0.04

COL1A1

Lower in old

0.71 ± 0.11

0.02 ± 0.02

0.0003

MMP1

Lower in old

0.28 ± 0.17

0.07 ± 0.03

0.03

MMP13

Lower in old

0.08 ± 0.12

0.02 ± 0.02

0.28

ADAMTS-4

Lower in old

0.07 ± 0.05

0.03 ± 0.02

0.07

IL1β

Lower in old

0.0001 ± 0.0002

2.66 × 10-5 ± 1.14 × 10-5

0.03

TNFα

No change

0.0001 ± 7.7 × 10-5

9.8 × 10-5 ± 4.5 × 10-5

0.4

TBFβ

No change

0.58 ± 0.14

1.06 ± 0.5

0.12


Values for real-time polymerase chain reaction (RT-PCR) are the mean ± standard deviation of relative expression levels normalised to expression of glyceraldehyde-3-phosphate dehydrogenase. Statistical significance was tested using Student's t test. DKK1, dickkopf homolog 1; COL10, collagen type X; RUNX2, Runt-related transcription factor 2; SRPX, Sushi repeat-containing protein; ACSL5, acyl-CoA synthetase long-chain family member 5; IL7R, interleukin 7 receptor; COL2A1, collagen type II, alpha 1; COL1A1, collagen type I, alpha 1; MMP1, matrix metalloproteinase 1; MMP-13, matrix metalloproteinase 13; ADAMTS4, a disintegrin and metalloproteinase with thrombospondin motifs 4; IL-1β, interleukin 1 beta; TNFα, tumour necrosis factor alpha; TGFβ, transforming growth factor beta.

Peffers et al. Arthritis Research & Therapy 2013 15:R98   doi:10.1186/ar4278

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