Preparation of fHAs. High molecular weight (HMW) hyaluronic acid (HA) was digested with bovine testis hyaluronidase. The resulting fragments were separated on a Bio Gel P10 column. For determination of fragment size fluorophore-assisted carbohydrate electrophoresis (FACE) analysis was performed. In this example, fractions 18, 23, 42, 48 and 64 derived from one HA-preparation were compared with a commercially-available HA standard (Sigma-Aldrich). M, HA standard marker; ds, disaccharide units.
Quero et al. Arthritis Research & Therapy 2013 15:R94 doi:10.1186/ar4274