Cartilage degradation induced by interleukin 1β and tumor necrosis factor α in mouse fetal metatarsals can be blocked by small molecule WNT inhibitors. (A) Morphological changes of metatarsals caused by interleukin 1β (IL-1β) and tumor necrosis factor α (TNFα) (10 ng/ml each) can be blocked by cotreatment with PKF115-584 at a concentration of 1.0 µM. CGP049090 partially blocks resorption of the metatarsals, whereas PKF118-310 did not have an effect. A representative picture of three independent experiments is shown. Scale bar represents 500 µm. (B) PKF115-584 dose-dependently blocked a decrease in bone length caused by IL-1β/TNFα treatment (10 ng/ml each) over time (indicated by red arrow). Other compounds and other concentrations did not counteract detrimental effects of IL-1β/TNFα treatment. Data represent the means of three independent experiments with 95% confidence intervals.
Landman et al. Arthritis Research & Therapy 2013 15:R93 doi:10.1186/ar4273