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Open Access Research article

Hypoxia promotes redifferentiation and suppresses markers of hypertrophy and degeneration in both healthy and osteoarthritic chondrocytes

Brandon D Markway, Holly Cho and Brian Johnstone*

Author Affiliations

Department of Orthopaedics & Rehabilitation, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, OP31, Portland, OR 97239 USA

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Arthritis Research & Therapy 2013, 15:R92  doi:10.1186/ar4272

Published: 21 August 2013

Additional files

Additional file 1:

Macroscopic and toluidine blue-stained sections of healthy and osteoarthritic cartilage specimens. Representative cartilage specimen images of healthy (A) and (C) and OA (B) and (D) are shown.

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Additional file 2:

Ratio of COL2A1 to COL1A1 expression in healthy and osteoarthritic chondrocytes. The ratios of the relative gene expression levels of COL2A1 and COL1A1 in pellet cultures following 2 wk of redifferentiation in either 20% oxygen (gray bars) or 2% oxygen (white bars) were calculated. All values are the mean ratios from n = 5 donors. Error bars represent 1 SD. Statistical significance was determined by performing independent t-tests (between disease conditions) and paired t-tests (between oxygen levels). *P < 0.05.

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Additional file 3:

Full hypoxia-inducible factor 2α blot for healthy chondrocytes shown in Figure 7. Unmodified version of healthy chondrocyte HIF-2α blot shown in Figure 7.

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Open Data

Additional file 4:

Full hypoxia-inducible factor 2α blot for osteoarthritic chondrocytes shown in Figure 7. Unmodified version of OA chondrocyte HIF-2α blot shown in Figure 7.

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Open Data