Hypoxia promotes redifferentiation and suppresses markers of hypertrophy and degeneration in both healthy and osteoarthritic chondrocytes
Department of Orthopaedics & Rehabilitation, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, OP31, Portland, OR 97239 USA
Arthritis Research & Therapy 2013, 15:R92 doi:10.1186/ar4272Published: 21 August 2013
Additional file 1:
Macroscopic and toluidine blue-stained sections of healthy and osteoarthritic cartilage specimens. Representative cartilage specimen images of healthy (A) and (C) and OA (B) and (D) are shown.
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Additional file 2:
Ratio of COL2A1 to COL1A1 expression in healthy and osteoarthritic chondrocytes. The ratios of the relative gene expression levels of COL2A1 and COL1A1 in pellet cultures following 2 wk of redifferentiation in either 20% oxygen (gray bars) or 2% oxygen (white bars) were calculated. All values are the mean ratios from n = 5 donors. Error bars represent 1 SD. Statistical significance was determined by performing independent t-tests (between disease conditions) and paired t-tests (between oxygen levels). *P < 0.05.
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Additional file 3:
Full hypoxia-inducible factor 2α blot for healthy chondrocytes shown in Figure 7. Unmodified version of healthy chondrocyte HIF-2α blot shown in Figure 7.
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Additional file 4:
Full hypoxia-inducible factor 2α blot for osteoarthritic chondrocytes shown in Figure 7. Unmodified version of OA chondrocyte HIF-2α blot shown in Figure 7.
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