Figure 3.

Expression of focal adhesion molecules in dermal blood microvascular endothelial cells and lymphatic microvascular endothelial cells. Representative immunofluorescent images of focal adhesion molecules in blood microvascular endothelial cells (B-MVECs) (A) and lymphatic microvascular endothelial cells (Ly-MVECs) (B) cultured with healthy sera are shown. Representative immunofluorescent photomicrographs show double-labeling for αvβ3 integrin (green) (a), phosphorylated focal adhesion kinase (FAK) (red) (b) and a merged image (c). Representative immunofluorescent photomicrographs show double-labeling for phalloidin (green) (d), vinculin (red) (e) and a merged image (f). 4′,6-diamidino-2-phenylindole (DAPI)-stained nuclei are blue in merged images. In both B-MVECs and Ly-MVECs, αvβ3 integrin (a) and phosphorylated FAK (b) are co-localized. αvβ3 integrin (a), phosphorylated FAK (b) and vinculin (e) are visible as small dots distributed all over the cell surface. Phalloidin-stained actin filaments (d) are arranged in long strands running parallel to cell borders. Original magnification, ×40.

Villano et al. Arthritis Research & Therapy 2013 15:R90   doi:10.1186/ar4270
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