Immunostaining of synovial macrophages. A-D, Representative sections of synovial membranes stained with a monoclonal anti-rabbit macrophage antibody (RAM11) from healthy rabbits (A), high fat diet (HFD) rabbits (B), chronic antigen-induced arthritis (AIA) rabbits (C) and HFD + AIA rabbits (D), original magnifications x100, scale bar = 100 µm. E, Densitometric analysis of RAM11 staining percentage in the synovium of each group of animals. Bars show the mean and SEM (n = 7 to 9 rabbits per group). F, Detail of the RAM11-positive cells observed in AIA synovium (left side) and in the HFD + AIA synovial membrane (right side), original magnifications x630, scale bar = 25 µm. RAM11-positive foam cells of increased size, some of them with three nuclei (white arrow), are present in the HFD + AIA synovium.
Prieto-Potín et al. Arthritis Research & Therapy 2013 15:R81 doi:10.1186/ar4261