Hypercholesterolemia boosts joint destruction in chronic arthritis. An experimental model aggravated by foam macrophage infiltration
Bone and Joint Research Unit, Service of Rheumatology, IIS Fundación Jiménez Díaz, Universidad Autónoma, Av. Reyes Católicos 2, 28048, Madrid, Spain
Arthritis Research & Therapy 2013, 15:R81 doi:10.1186/ar4261Published: 13 August 2013
Additional file 1:
High fat diet (HFD) and antigen induced arthritis (AIA) interventions increase TNF-α (A) and MCP-1 (B) gene expression in the synovium. Gene expression was examined by real time PCR and results are expressed as fold induction. Bars show the mean and SEM (n = 7 to 9 rabbits per group).
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Additional file 2:
RANKL and OPG protein expression. A, C and E, Densitometric analysis of receptor activator nuclear kappa B ligand (RANKL) and osteoprotegerin (OPG) protein expression in cartilage (A), subchondral bone (C) and synovium (E). Bars show the mean and SEM (n = 7 to 9 rabbits per group). B, D and F, Representative Western blot images for RANKL and OPG detection in cartilage (B), subchondral bone (C) and synovium (F). EZ Blue-stained gels used as protein loading controls are also shown for healthy rabbits, high fat diet (HFD) rabbits, chronic antigen induced arthritis (AIA) rabbits and HFD + AIA rabbits.
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