Table 1

Phenotypic characterization of adipose-derived stem cells through immunophenotype and RT-PCR

Analyzed marker

Immunophenotypea

RT-PCRb


Positivec

   CD73

NT

++

   CD90

NT

++

   CD105

NT

++

   CD166

NT

++

   CD271

++

++

   MSCA

++

NT

Negativec

   CD14

NT

+

   CD34

NT

-

   CD45

+

-

   CD117

NT

-

GAPDHd

NT

++


aNT, not tested; +, positive (≤50%); ++, positive (≥85%). bNT, not tested; -, negative; +, positive (faint band); ++, positive (intense band). cExpected result of surface antigen expression on mesenchymal stem cells according to the literature [22,23]. dHousekeeping gene.

Garza-Veloz et al. Arthritis Research & Therapy 2013 15:R80   doi:10.1186/ar4260

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