Loss of phosphorylation of both LKB1 and AMPKα in human knee osteoarthritic chondrocytes. Lysates of cultured primary human knee chondrocytes (grade I to III) were subjected to western blot analysis with antibodies to phospho-liver kinase B1 (p-LKB1; Ser428) and phospho-AMP-activated protein kinase alpha (p-AMPKα; Thr172), as described in Materials and methods. Data representative of three individual experiments (total of 15 different donors).
Petursson et al. Arthritis Research & Therapy 2013 15:R77 doi:10.1186/ar4254