Percentage of indoleamine 2,3-dioxygenase (IDO)- and Foxp3-expressing peripheral blood cells in patients with primary Sjögren's syndrome (pSS). (A,B) Control of fluorescein isothiocyanate (FITC)-labeled-rabbit anti-sheep specificity staining. (C) An electronic gate was made for CCR6+ cells. (D) From the gate C CCR6+⁄CD123hi cells were determined. (E) From the latter CCR6+⁄CD123hi⁄IDO+ cells were defined. (F) An electronic gate was made for CD4+ cells. (G) From the gate F CD4+⁄CD25hi were determined and an electronic gate was made for double positive cells. (H) From the latter CD4+⁄CD25hi⁄Foxp3+ cells were defined. Bar graphs show percentage of (I) CCR6+⁄CD123hi⁄IDO+ cells, (J) CD4+⁄CD25hi⁄Foxp3+ cells, (K) CD8+⁄CD28-⁄Foxp3+ cells. A total of 100,000 to 250,000 events were recorded for each sample before any gate setting, and were analyzed with the CellQuestPro software (BD Biosciences). (I-K) Results are expressed as median, 10th, 25th, 75th, and 90th percentiles. *P < 0.05.
Furuzawa-Carballeda et al. Arthritis Research & Therapy 2013 15:R68 doi:10.1186/ar4245