Percentage of immature/transitional IL-10-producing B peripheral-cell subtype in patients with primary Sjögren's syndrome (pSS). CD19+ blood B cells were obtained by positive selection with microbeads. (A) Representative contour plot of CD19+ B cells. An electronic gate was made for CD38hi cells. (B) From the gate A CD19+⁄CD38hi⁄CD24hi cells were determined. (C) From the gate B CD19+⁄CD38hi⁄CD24hi⁄CD20+ were defined. (D) From the latter CD19+⁄CD38hi⁄CD24hi⁄CD20+⁄IL-10+ cells were determined. Bar graphs show percentage of (E) CD19+⁄CD38hi⁄CD24hi⁄CD5+⁄IL-10+, (F) CD19+⁄CD38hi⁄CD24hi⁄CD10+⁄IL-10+, (G) CD19+⁄CD38hi⁄CD24hi⁄CD20+⁄IL-10+, (H) CD19+⁄CD38hi⁄CD24hi⁄CD27-⁄IL-10+, (I) CD19+⁄CD38hi⁄CD24hi⁄CXCR4+⁄IL-10+-, and (J) CD19+⁄CD38hi⁄CD24hi⁄CXCR7+⁄IL-10+-producing B peripheral cells. A total of 50,000 to 100,000 events were recorded for each sample before any gate setting and were analyzed with the CellQuestPro software (BD Biosciences). (E-J) Results are expressed as median, 10th, 25th, 75th, and 90th percentiles. *P < 0.05.
Furuzawa-Carballeda et al. Arthritis Research & Therapy 2013 15:R68 doi:10.1186/ar4245