Standard curve for IgG1. A standard curve was made using Magellan Data Analysis Software to quantify the A450 nm values from ELISA. An ELISA plate was coated with a known serial dilution (0.0625-4 µg/ml) of native IgG1 subclass from human myeloma plasma before incubating wells with HRP-conjugated secondary antibody. Correlation between A450 nm values and concentration was fitted by the regression output (r = 0.99507).
Carlsen et al. Arthritis Research & Therapy 2013 15:R61 doi:10.1186/ar4234