SDS-PAGE gel of bacterial and human HSP60. Size and purity of each recombinant protein were evaluated using 10% SDS-PAGE gel. The different HSP60 proteins for ELISA were purified using a Ni+ column and imidazole as a competing reagent. Vector transformation was confirmed by isopropyl-β-D-thiogalactopyranoside (IPGT) induction. Lane A: Molecular weight marker. Lanes B, E, H and K: Purified HSP60. Lanes C and D, F and G, I and J and L and M: Total protein content from Escherichia coli strain BL21 (DE3) with (+) and without (-) IPGT induction.
Carlsen et al. Arthritis Research & Therapy 2013 15:R61 doi:10.1186/ar4234