Additional Figure 1.

Mig6 expression, EGFR activation and proliferation in ligament and bone. Sections of the center of normal six-week old Mig-6-flox knees (A, C, E) and Mig-6-flox;Prx1Cre conditional knockout (Mig-6-cko) knees (B, D, F) immunostained with antibodies against Mig-6 (A, B); phosphorylated EGFR (p-EGFR, C, D); or Ki67 (E, F). (A, B) Endogenous localization of Mig-6 protein in ligament (arrows in A) and bone (arrowheads in A) which is absent in the knockout animal (B). (C-F) EGFR signaling and proliferation are occurring within the ligament especially the ligament/cartilage junction (arrows), and within the subchondral bone (arrowheads), of the normal Mig-6-flox knee (C, E), and EGFR signaling and proliferation are increased in these tissues in the Mig-6-cko knee (compare D, F to C, E). Scale Bar = 100 μm.

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Shepard et al. Arthritis Research & Therapy 2013 15:R60   doi:10.1186/ar4233