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Open Access Research article

Transient anabolic effects accompany epidermal growth factor receptor signal activation in articular cartilage in vivo

John B Shepard1, Jae-Wook Jeong2, Nita J Maihle3, Sean O'Brien1 and Caroline N Dealy14*

Author Affiliations

1 Center for Regenerative Medicine and Skeletal Development, Department of Reconstructive Sciences, School of Dental Medicine, University of Connecticut Health Center, 263 Farmington Avenue, Farmington CT 06030, USA

2 Departments of Obstetrics, Gynecology and Reproductive Biology, Michigan State University, 333 Bostwick Avenue NE, Grand Rapids, MI 49503, USA

3 Departments of Obstetrics and Gynecology, Pathobiology, and Pharmacology, Yale University, 310 Cedar Street, New Haven, CT 06520, USA

4 Department of Orthopaedic Surgery, School of Medicine, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA

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Arthritis Research & Therapy 2013, 15:R60  doi:10.1186/ar4233

Published: 25 May 2013

Additional files

Additional Figure 1:

Mig6 expression, EGFR activation and proliferation in ligament and bone. Sections of the center of normal six-week old Mig-6-flox knees (A, C, E) and Mig-6-flox;Prx1Cre conditional knockout (Mig-6-cko) knees (B, D, F) immunostained with antibodies against Mig-6 (A, B); phosphorylated EGFR (p-EGFR, C, D); or Ki67 (E, F). (A, B) Endogenous localization of Mig-6 protein in ligament (arrows in A) and bone (arrowheads in A) which is absent in the knockout animal (B). (C-F) EGFR signaling and proliferation are occurring within the ligament especially the ligament/cartilage junction (arrows), and within the subchondral bone (arrowheads), of the normal Mig-6-flox knee (C, E), and EGFR signaling and proliferation are increased in these tissues in the Mig-6-cko knee (compare D, F to C, E). Scale Bar = 100 μm.

Format: TIFF Size: 10.3MB Download file

Open Data

Additional Figure 2:

Mig6 expression, EGFR activation and proliferation in menisci and/or synovium. Sections of the menisci of normal six-week-old Mig-6-flox knees (A, C, E) and Mig-6-flox;Prx1Cre conditional knockout (Mig-6-cko) knees (B, D, F) immunostained with antibodies against Mig-6 (A, B); phosphorylated EGFR (p-EGFR, C, D); or Ki67 (E, F). (A, B) Endogenous localization of Mig-6 protein in the superficial region of the meniscus (arrowheads in A) which is absent in the knockout animal (B). (C-F) EGFR signaling and proliferation are occurring within the superficial region of the meniscus (arrowheads) of the normal Mig-6-flox knee (C, E), and EGFR signaling and proliferation is enhanced and extends further into the meniscus in the Mig-6-cko knee (compare D, F to C, E). In addition, immunostaining for pEGFR is also present in the thickened Mig-6-cko synovial tissue (arrow in D). Scale Bar = 100 μm.

Format: TIFF Size: 4.8MB Download file

Open Data