Histological sections following conventional and immunohistological staining for the detection of cartilage matrix molecules. Proteoglycan content was assessed in fresh and cultured (+/- TGF-β1) cartilage, as well as in the BNC by safranin-O staining (A); aggrecan neosynthesis was shown using an antibody directed against a neoepitope on aggrecan molecules (B). In addition, specific staining for collagen type II (C) and type I (D) was performed. Magnification 40 x. BNC, bacterial nana-cellulose; TGF-β1, transforming growth factor-β1.
Pretzel et al. Arthritis Research & Therapy 2013 15:R59 doi:10.1186/ar4231