Adenosine A2A receptor occupancy increases CTGF mRNA and protein expression and secretion. NHDF cells were incubated with CGS-26180 at 1 µM A) for 4 hours for CTGF mRNA analysis or B) for 24 hours prior to cellular fractionation and protein concentration from supernates as described under "Methods". Bands were quantified and data represent means ± S.E.M. from three to eight independent experiments. Statistics were performed by one-way ANOVA with post-hoc analysis by Newman-Keuls test, *P <0.05 vs. non-stimulated control.
Chan et al. Arthritis Research & Therapy 2013 15:R58 doi:10.1186/ar4229