Adenosine A2A receptor occupancy suppresses Fli1 mRNA expression in human dermal fibroblasts. NHDF cells were incubated with CGS-26180 at A) 10 µM at the indicated time-points or B) 1 µM or 10 µM for four or eight hours. C) Incubation with the A2A receptor-selective antagonist ZM241385 (1 µM) prior to CGS-26180 (10 µM) for four hours prevents the decrease on Fli1 expression. Data represent means ± S.E.M. of four to eight independent experiments. *P <0.05 vs. non-stimulated control (One-way ANOVA, post-hoc analysis by Newman-Keuls test); ###P <0.001, CGS-26180 + ZM241385 vs. CGS-26180 (unpaired t-test).
Chan et al. Arthritis Research & Therapy 2013 15:R58 doi:10.1186/ar4229