Additional file 1, Figure S1.

Representative HPLC chromatograms of the purification and analysis of [18F]fluoro-PEG-folate. (A) Semi prep HPLC chromatogram of the purification of [18F]fluoro-PEG-folate; top: UV detection, bottom: radioactivity detection. The product elutes at 31 to 33 minutes, radiochemical yield, calculated from this chromatogram is 73%. The non-radioactive reference compound eluted at the same retention time as the radioactive compound (result not shown) (B) Analysis (quality control) of the final solution of [18F]fluoro-PEG-folate; top: UV detection, bottom: radioactivity detection. Radiochemical purity >99%. (C) Analysis (quality control) of the final solution of [18F]fluoro-PEG-folate, with the addition of reference compound; top: UV detection, bottom: radioactivity detection. The reference compound elutes at the same time as [18F]fluoro-PEG-folate and confirms the identity of [18F]fluoro-PEG-folate.

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Gent et al. Arthritis Research & Therapy 2013 15:R37   doi:10.1186/ar4191