Open Access Research article

Evaluation of the novel folate receptor ligand [18F]fluoro-PEG-folate for macrophage targeting in a rat model of arthritis

Yoony YJ Gent1, Karin Weijers1, Carla FM Molthoff2, Albert D Windhorst2, Marc C Huisman2, Desirée EC Smith3, Sumith A Kularatne4, Gerrit Jansen1, Philip S Low4, Adriaan A Lammertsma2 and Conny J van der Laken1*

Author Affiliations

1 Department of Rheumatology, VU University Medical Center, De Boelelaan 1117, Amsterdam, 1081 HV, The Netherlands

2 Department of Radiology & Nuclear Medicine, VU University Medical Center, De Boelelaan 1117, Amsterdam, 1081 HV, The Netherlands

3 Department of Clinical Chemistry, VU University Medical Center, De Boelelaan 1117, Amsterdam, 1081 HV, The Netherlands

4 Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA

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Arthritis Research & Therapy 2013, 15:R37  doi:10.1186/ar4191

Published: 1 March 2013

Additional files

Additional file 1, Figure S1:

Representative HPLC chromatograms of the purification and analysis of [18F]fluoro-PEG-folate. (A) Semi prep HPLC chromatogram of the purification of [18F]fluoro-PEG-folate; top: UV detection, bottom: radioactivity detection. The product elutes at 31 to 33 minutes, radiochemical yield, calculated from this chromatogram is 73%. The non-radioactive reference compound eluted at the same retention time as the radioactive compound (result not shown) (B) Analysis (quality control) of the final solution of [18F]fluoro-PEG-folate; top: UV detection, bottom: radioactivity detection. Radiochemical purity >99%. (C) Analysis (quality control) of the final solution of [18F]fluoro-PEG-folate, with the addition of reference compound; top: UV detection, bottom: radioactivity detection. The reference compound elutes at the same time as [18F]fluoro-PEG-folate and confirms the identity of [18F]fluoro-PEG-folate.

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Additional file 2, Figure S2:

Uptake [18F]fluoro-PEG-folate in arthritic knee and blood. (A) Time-activity curves of [18F]fluoro-PEG-folate uptake in arthritic knee and blood. (B) Arthritic knee-to-blood ratio as function of time. Results are presented as mean ± SD of four arthritic rats.

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Additional file 3, Figure S3:

[18F]fluoro-PEG-folate imaging of arthritic rats. [18F]fluoro-PEG-folate uptake in the right arthritic knee at (A) baseline (arrow) and (B) after blocking the FR with an excess dose of glucosamine-folate. In the latter case abolishment of the signal in the arthritic knee is evident.

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