Changes in B cell subsets during induction of remission and sequential regulatory T cell (Treg) transfer. B cell subsets were analyzed by flow cytometry in the peripheral blood during induction of remission and after an additional adoptive transfer of 1.5 × 106 Treg/mouse (Treg) and compared to age-matched mice that received only glucocorticosteroid (GC)/cyclophosphamide (CTX) and PBS (Control). (A) Representative dot plots show expression of IgD among CD19+ B cells and the percentage of CD19+CD138+ plasma blasts (PB) and of CD19-CD138+ plasma cells (PC) among lymphocytes, respectively. (B-D) Average percentage of IgD+ cells among CD19+ B cells (B), average percentage of CD19+CD138+ PB among lymphocytes (C) and average percentage of CD19-CD138+ PC among lymphocytes (D) at the indicated time points during the study. Data are the summary of two independent experiments with six to eight mice per group in total. Error bars indicate standard error of the mean (SEM).
Weigert et al. Arthritis Research & Therapy 2013 15:R35 doi:10.1186/ar4188