Changes in regulatory T cell (Treg) and conventional T cell (Tcon) subsets during induction of remission and sequential Treg transfer. Treg and Tcon subsets were analyzed by flow cytometry in the peripheral blood of (NZBxNZW) F1 mice during induction of remission and after an additional adoptive transfer of 1.5 × 106 Treg/mouse (Treg) and compared to age-matched mice that received only glucocorticosteroid (GC)/cyclophosphamide (CTX) and PBS (Control). (A) Representative dot plots show expression of CD25 and CD44 among CD4+ gated Foxp3+ Treg and Foxp3- Tcon. (B) Average percentage of Foxp3+ Treg among CD4+ T cells at the indicated time points during the course of the study (*P <0.05; Treg vs Control). Bar diagrams compare the average percentage of Foxp3+ Treg among CD4+ T cells before (at day -4) and after the Treg transfer (at day 14) within the respective group (*P <0.05; before vs after). (C) Average percentage of CD25+ cells and CD44hi cells among CD4+Foxp3+ Treg at the indicated time points during the study. (D) Average percentage of CD25+ cells and CD44hi cells among CD4+Foxp3- Tcon throughout the study. (B-D) Data are the summary of two independent experiments with six to eight mice per group in total. Error bars indicate standard error of the mean (SEM).
Weigert et al. Arthritis Research & Therapy 2013 15:R35 doi:10.1186/ar4188