Additional file 3.

mRNA expression of CD68, cadherin 11 and IL-6. (a) Fold change in mRNA expression of the macrophage surface marker CD68 in K/BxN fibroblast-like synoviocytes (FLS) at passages 3 and 4 determined by real-time RT-PCR. For each gene product data were normalized for levels of the housekeeping gene 18S rRNA and are presented as fold change in expression (± standard error) relative to the P2 control. Results shown are the combined duplicates of two K/BxN FLS. (b) mRNA ΔCt values for cadherin 11 in three separate wild-type non-inflamed and three K/BxN FLS cell lines after 16 hr treatments with either vehicle or TNF-α at 10 ng/ml. (c) Fold change in IL-6 mRNA expression over 0, 2, 4, 8, 16 and 24 hr following treatment with either corticosterone (100 nmol/l) or TNF-α (10 ng/ml). Results shown are the combined duplicates of three separate FLS lines. Data were normalized for levels of the housekeeping gene 18S rRNA and are presented as fold change in expression (± standard error) relative to the 0 hr control. *P < 0.05, **P < 0.001 versus respective untreated control.

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Hardy et al. Arthritis Research & Therapy 2013 15:R24   doi:10.1186/ar4158