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This article is part of the supplement: Proceedings of the 8th Global Arthritis Research Network (GARN) Meeting and 1st Bio-Rheumatology International Congress (BRIC)

Poster presentation

Dendritic cell immunoreceptor (DCIR) is associated with anti-cyclic citrullinated peptides (anti-CCP) antibody - negative rheumatoid arthritis in Chinese Han population

Jianping Guo, Xinyu Wu, Xiaolan Lu, Yin Su, Ru Li, Jing He, Xu Liu and Zhanguo Li*

  • * Corresponding author: Zhanguo Li

Author Affiliations

Department of Rheumatology and Immunology, Peking University People's Hospital, Beijing, 100044, China

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Arthritis Research & Therapy 2012, 14(Suppl 1):P22  doi:10.1186/ar3623

The electronic version of this article is the complete one and can be found online at: http://arthritis-research.com/content/14/S1/P22


Published:9 February 2012

© 2012 Guo et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Background

The dendritic cell immunoreceptor (DCIR) is an important member of C-type lectin superfamily, which has been shown evidence for susceptibility to arthritis in multiple animal models. The human DCIR polymorphisms have been shown a nominal association with rheumatoid arthritis (RA) susceptibility, mainly with anti-cyclic citrullinated peptides (anti-CCP) antibody -negative RA in Swedish population. We aimed to investigate the possible association of DCIR with RA susceptibility in Chinese Han population.

Methods

A total of 1193 patients with RA and 1278 healthy controls were genotyped for single-nucleotide polymorphism (SNP) rs2377422 and rs10840759. Association analyses were performed on the whole data set and on RA subsets based on the status of anti-CCP antibody in RA patients. The interaction between rs2377422 and HLA-DRB1 shared epitope (SE) was also analyzed for RA susceptibility. Finally, we carried out association analysis of rs2377422 with DCIR mRNA expression in RA patients.

Results

The DCIR rs2377422 was found significantly associated with RA (allele analysis: OR 1.17, 95%CI 1.04-1.31, p=3.67 × 10-3; genotype analysis (recessive model C/C vs. T/T + T/C): OR 1.37, 95%CI 1.08-1.73, p=9.04 × 10-3). Following stratification for anti-CCP status, a suggestive association of rs2377422 with anti-CCP-positive RA was observed (p = 0.058, OR 1.34, 95%CI 0.99-1.82). In contrast, the CC genotype of rs2377422 was found specifically to confer susceptible risk for anti-CCP-negative RA (OR 1.92, 95%CI 1.27-2.90, p=1.99 × 10-3), despite loss of power in the analysis. The relative risk of RA was 3.0 (95%CI 1.33-6.91, p=6.48 × 10-3) in individuals carrying rs2377422 TT genotype with SE alleles, and 9.06 (95%CI 3.33-25.61, p=2.08 × 10-6) in individuals carrying rs2377422 CC genotype with SE genes. The interaction between rs2377422 and SE alleles was significant, as measured by the attributable proportion (AP) due to interaction (0.60). DCIR gene transcription quantification analysis further proved the dominant effect of rs2480256 CC genotype on DCIR expression levels in RA patients (C/C vs. T/T + T/C: 0.55 ± 0.09 vs. 0.24 ± 0.02, p=1.67 × 10-3).

Conclusions

Our study provides evidence for association between DCIR rs2377422 and RA, particularly with anti-CCP-negative RA in non-Caucasian populations.