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This article is part of the supplement: Proceedings of the 8th Global Arthritis Research Network (GARN) Meeting and 1st Bio-Rheumatology International Congress (BRIC)

Poster presentation

Balb/c FasKO mice develop allergic blepharitis associated with hyper-production of IgE

Ayumi Fukuoka1*, Shizue Yumikura-Futatsugi2, Suzuka Takahashi13, Hirotaka Kazama1, Kenji Nakanishi2 and Shin Yonehara1

  • * Corresponding author: Ayumi Fukuoka

Author Affiliations

1 Graduate School of Biostudies, Kyoto University, Japan

2 Department of Immunology and Medical Zoology, Hyogo College of Medicine, Japan

3 Institute of Genome Reserch, The University of Tokushima, Japan

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Arthritis Research & Therapy 2012, 14(Suppl 1):P19  doi:10.1186/ar3620

The electronic version of this article is the complete one and can be found online at: http://arthritis-research.com/content/14/S1/P19


Published:9 February 2012

© 2012 Fukuoka et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Poster presentation

Fas is a member of the TNF receptor family and crucial for induction of apoptosis. MRL- lpr/lpr mice, which carry a mutation of Fas, spontaneously develop systemic autoimmune disease including arthropathy, indicating that Fas plays an important role in elimination of self-reactive immunocytes by apoptosis. In addition to autoimmune diseases, we found a novel phenotype of FasKO mice exclusively in Balb/c genetic background that is allergic blepharitis. Allergic blepharitis is revealed in Balb/c FasKO mice from 15 week-old and about 85% of the mice suffered from allergic blepharitis at 35 week-old. Serum concentrations of both IgG1 and IgE Abs were about 100-times higher in 20-week old FasKO mice than in WT mice; however, there was no significant difference between WT and FasKO mice in the ability of B cells to produce IgG1 and IgE Abs in the presence of IL-4 and anti-CD40 Ab inducing co-stimulatory signals. Additionally, the production of IL-4 by T cells was same. These results suggested that other type of cells enhanced IgG1 and IgE Abs production from B cells in Balb/c FasKO mice. To identify the cells enhancing IgG1 and IgE Abs production, we cultured B cells in vitro in the presence of IL-4 and anti-CD40 Ab together with various types of cells from Balb/c FasKO mice. In the result, we found FasKO non-T non-B cells upregulated the production of both IgG1 and IgE from B cells. Moreover, the number of these cells was specifically increased in Balb/c FasKO mice.All the results indicate that these cells enhance production of IgG1 and IgE from B cells in the presence of IL-4 and anti-CD40 Ab, and excessive accumulation of these cells may cause allergy via hyper-production of IgE.

thumbnailFigure 1. Balb/c FasKO mice develope allergic blepharitis.

References

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