Additional file 1.
IgG and C3 deposition in ankles of K/BxN mice lacking C3 or C5 and lacking FcR©. Deposition of IgG (red, left panels) and C3 (green, right panels) was determined by immunofluorescent microscopy in K/BxN mice expressing or not expressing C3, FcR©, and C5, as indicated in the left column. The key findings are that IgG is deposited in each of the joints. As expected, C3 is absent in the C3-deficient animal (A, lower right panel). Despite having decreased arthritis severity, C3 is still detectable in the joints of the C5/FcR©-deficient mouse (B, lower right panel). Slides were counterstained with DAPI (blue) to detect nuclei. Original objective: 40x.
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Auger et al. Arthritis Research & Therapy 2012 14:R269 doi:10.1186/ar4117