Table 1

Primer sequences

Gene

Sequence

Size

ACAN

Forward (6708-6727): TGA GTC CTC AAG CCT CCT GT

185 bp

Reverse (6873-6892): CCT CTG TCT CCT TGC AGG TC

ALP

Forward (1397-1416): CCA CGT CTT CAC ATT TGG TG

196 bp

Reverse(1573-1592): AGA CTG CGC CTG GTA GTT GT

COL10A1

Forward (1670-1690): AAT GCC TGT GTC TGC TTT TAC

130 bp

Reverse (1779-1799): ACA AGT AAA GAT TCC AGT CCT

COL2A1

Forward (459-478): ATT TCA AGG CAA TCC TGG TG

218 bp

Reverse (657-676): GGC CTG GAT AAC CTC TGT GA

GAPDH

Forward (113-133): TGA AGG TCG GAG TCA ACG GAT

181 bp

Reverse (273-293): TTC TCA GCC TTG ACG GTG CCA

OC

Forward (20-39): TGA GAG CCC TCA CAC TCC TC

151 bp

Reverse (170-151): CGC CTG GGT CTC TTC ACT AC

RUNX 2

Forward (1312-1331): CAG ACC AGC AGC ACT CCA TA

178 bp

Reverse (1470-1489): CAG CGT CAA CAC CAT CAT TC

SOX9

Forward (19-38): TTC ATG AAG ATG ACC GAC GA

175 bp

Reverse (174-193): CGC TCT CCT TCT TCA GAT CG

Antoniou et al. Arthritis Research & Therapy 2012 14:R267   doi:10.1186/ar4113