Effect of Link N on proteoglycan deposition by mesenchymal stem cells (MSCs) in serum-free medium. (A) Alcian blue staining of proteoglycan in extracellular matrix after MSCs were cultured in serum-free medium devoid of TGF-β (control), serum-free medium with 1 μg/ml Link N, and serum-free medium with 10 ng/ml TGF-β3 for 21 days. (B) The absorbance value of the solubilized Alcian blue at 620 nm. The results are shown as mean ± SD of three independent experiments with MSCs from three different donors.
Antoniou et al. Arthritis Research & Therapy 2012 14:R267 doi:10.1186/ar4113