Additional file 3.

Figure S2. Inflammasome-independent IL-1β transcription. (A) IL-1β mRNA expression levels (× 1000) in bone marrow-derived macrophages isolated from wild-type (WT), NLRP3-, ASC-, and caspase-1 gene-deficient mice. mRNA expression after 24 hours of stimulation with either medium or 5 × 10^6 B. burgdorferi per mL. At least five animals per group, bars represent mean ± SEM. IL-6 (B) and TNF-α (C) mRNA expression and protein production (in ng/mL for IL-6, and pg/mL for TNF-α, respectively) by bone marrow-derived macrophages isolated from WT, NLRP3-, ASC-, and caspase-1 gene-deficient mice. mRNA expression after 24 hours of stimulation with either medium or 5 × 10^6 B. burgdorferi per mL. At least five animals per group, bars represent mean ± SEM. ASC, apoptosis-associated speck-like protein containing a caspase recruitment domain (CARD); NLRP3, nucleotide oligomerization domain (NOD)-like receptor P3.

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Oosting et al. Arthritis Research & Therapy 2012 14:R247   doi:10.1186/ar4090