Additional file 2.

Figure S1. IL-1β mRNA expression is TLR2- and MyD88-dependent. (A) IL-1β mRNA expression levels (x 1000) in bone marrow-derived macrophages isolated from wild-type (WT), TLR2-, MyD88-, NOD1-, NOD2-, and RICK gene-deficient mice. mRNA expression after 24 hours of stimulation with either medium or 5 × 10^6 B. burgdorferi per mL. At least five animals per group, bars represent mean ± SEM. *P <0.05; Mann-Whitney U test, two-tailed. IL-6 (B) and TNF-α (C) mRNA expression and protein production (in ng/mL for IL-6, and pg/mL for TNF-α, respectively) by bone marrow-derived macrophages isolated from WT, TLR2-, MyD88-, NOD1-, NOD2-, and RICK gene-deficient mice. mRNA expression after 24 hours of stimulation with either medium or 5 × 10^6 B. burgdorferi per mL. At least five animals per group, bars represent mean ± SEM. MyD88, myeloid differentiation factor 88; Nod, nucleotide-binding oligomerization domain; RICK, serine-threonine protein kinase with a caspase activation and recruitment domain; TLR, Toll-like receptor.

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Oosting et al. Arthritis Research & Therapy 2012 14:R247   doi:10.1186/ar4090