Table 1

Analysis of surface phenotype of splenocytes

Cell Number/Spleen


B cells

Average

Vehicle

SEM Vehicle

Average PCI-32765

SEM PCI-32765

P-value


B220+

3.97E+07

1.13E+07

9.06E+06

2.76E+06

*

Activated, B220+CD69+

3.71E+06

9.33E+05

5.29E+05

9.19E+04

**

T1, B220+AA4.1+CD23-

1.64E+07

5.90E+06

2.95E+06

8.72E+05

*

T2, B220+AA4.1+CD23+

1.60E+07

3.42E+06

4.02E+06

1.79E+06

**

Marginal zone, B220+AA4.1-CD23-

1.99E+06

5.05E+05

5.25E+05

1.92E+05

*

Follicular, B220+AA4.1-CD23+

5.13E+06

1.89E+06

8.82E+05

2.18E+05

*

Plasmablast, B220+AA4.1-CD138+

3.62E+06

9.24E+05

4.36E+05

7.13E+04

**

Germinal center, B220+GL7+

8.61E+06

4.52E+06

8.72E+05

3.38E+05

NS

B1-like, B220+CD5+

1.01E+06

2.62E+05

2.60E+05

3.93E+04

*

Myeloid

Myeloid DC, CD11b+CD11c+

1.33E+07

2.76E+06

2.19E+06

4.28E+05

***

Macrophage, CD11b+F4/80+

1.32E+07

3.11E+06

2.15E+06

3.88E+05

**

CD11b+Ly6C+

1.74E+06

5.54E+05

3.40E+05

6.72E+04

*

Neutrophils, CD11b+Ly6G+

1.50E+07

3.51E+06

2.41E+06

4.16E+05

**

Mast cell, CD34+c-Kit+

3.74E+06

9.87E+05

7.36E+05

2.54E+05

*

T cells

CD4+

2.31E+07

4.00E+06

9.83E+06

9.76E+05

**

Activated, CD4+CD69+

8.29E+06

1.36E+06

2.64E+06

1.01E+06

***

Naïve, CD4+CD44-CD62L+

9.73E+05

2.34E+05

5.68E+05

9.45E+04

NS

Central memory, CD4+CD44+CD62L+

5.11E+06

7.89E+05

2.84E+06

3.49E+05

*

Effector memory, CD4+CD44+CD62L-

1.56E+07

3.04E+06

6.00E+06

7.58E+05

**

CD8+

2.01E+07

3.86E+06

5.29E+06

8.23E+05

**

Activated, CD8+CD69+

8.57E+06

1.60E+06

2.54E+06

1.17E+06

**

Naïve, CD8+CD44-CD62L+

6.18E+03

3.33E+03

3.34E+03

2.65E+03

NS

Central memory, CD8+CD44+CD62L+

5.64E+06

1.38E+06

9.08E+05

1.65E+05

**

Effector memory, CD8+CD44+CD62L-

1.45E+07

2.87E+06

4.38E+06

8.23E+05

**


Quantitative analysis of B6.Sle1.Sle3 splenic cell populations as determined by flow cytometry. All data are expressed as the mean (Average) and standard error of the mean (SEM) of the data for the PCI-32765-treated (n = 9) or vehicle-treated (n = 8) splenocytes, which were compared by two-tailed Student's t-test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.005, compared to vehicle; NS, not significant.

Hutcheson et al. Arthritis Research & Therapy 2012 14:R243   doi:10.1186/ar4086

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