Y27 treatment amelioration of lupus syndromes, and effects on serum antibody and renal histology in BDF1 mice. Chronic graft-versus-host disease (GVHD) was induced in recipient BDF1 mice by two intravenous injections of donor parental DBA/2 mice lymphocytes. Y27 (10, 20 and 40 mg/kg) was given from 3 days after the last cell injection, the control and model mice being given the same dose of vehicle (n = 7 per group). (A) Effect of treatment on mortality over 10 weeks (analyzed by log rank test) and urinary protein levels at the end of the experiment. Each symbol represents one mouse. Bars show the mean of the group. (B) Effect of treatment on blood urea nitrogen and triglyceride levels. (C) Anti-dsDNA antibodies and IgG1 levels decreased after Y27 treatment. (D) Y27 attenuated renal histopathology change in BDF1 mice (HE stain, 100×). Blinded scoring of glomerular alterations was done as indicated in Materials and methods. Bars show the mean ± standard error of the mean (SEM). **P < 0.01 versus control; #P < 0.05, ##P < 0.01 versus GVHD.
Xiao et al. Arthritis Research & Therapy 2012 14:R235 doi:10.1186/ar4078