Additional file 1.
CD4 and CD8 representative flow cytometry in lacrimal glands (LGs). Representative flow-cytometry plots of freshly isolated cells from LGs of CD25KO, γDKO, and IFN-γKO mice stained with either CD4-FITC-conjugated antibody (x axis) or CD8-PE-conjugated antibody (y axis) at ages 8, 12, and 16 weeks (8W,12W,16W, respectively). Lymphocytes were gated based on characteristic light-scatter properties; single lymphocytes were gated based on forward-scatter height versus forward-scatter area (FSC-A), and dead cells were excluded with propidium iodide staining.
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Pelegrino et al. Arthritis Research & Therapy 2012 14:R234 doi:10.1186/ar4077