Open Access Research article

Deletion of interferon-γ delays onset and severity of dacryoadenitis in CD25KO mice

Flavia SA Pelegrino, Eugene A Volpe, Niral B Gandhi, De-Quan Li, Stephen C Pflugfelder and Cintia S de Paiva*

Author Affiliations

Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030, USA

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Arthritis Research & Therapy 2012, 14:R234  doi:10.1186/ar4077

Published: 1 November 2012

Additional files

Additional file 1:

CD4 and CD8 representative flow cytometry in lacrimal glands (LGs). Representative flow-cytometry plots of freshly isolated cells from LGs of CD25KO, γDKO, and IFN-γKO mice stained with either CD4-FITC-conjugated antibody (x axis) or CD8-PE-conjugated antibody (y axis) at ages 8, 12, and 16 weeks (8W,12W,16W, respectively). Lymphocytes were gated based on characteristic light-scatter properties; single lymphocytes were gated based on forward-scatter height versus forward-scatter area (FSC-A), and dead cells were excluded with propidium iodide staining.

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