Table 1

Primers used for real-time qPCR.

Gene

Primer sequences

Accession number


18S rRNA

olg1, 5'-CCCCATGAACGAGGGAATT-3' olg2, 5'-GGGACTTAATCAACGCAAGCTT-3'

NR_003286

GAPDH

olg3, 5'-CATGAGAAGTATGACAACAGCCT-3' olg4, 5'-AGTCCTTCCACGATACCAAAGT-3'

NM_002046

ACTA2 (a-SMA)

olg20, 5'-CAGGGCTGTTTTCCCATCCAT-3' olg21, 5'-GCCATGTTCTATCGGGTACTTC-3'

NM_001613

COL1A1

olg149, 5'-GCTGGTGTGATGGGATTC-3' olg150, 5'-GGGAACACCTCGCTCT-3'

NM_000088

AdipoR1

olg396, 5'-TGACTGGCTAAAGGACAACG-3' olg397, 5'-AAAAGAGAAACAGCACGAAACC-3'

NM_015999

AdipoR2

olg400, 5'-CAGCCATTATAGTCTCCCAGTG-3' olg401, 5'-CCGAGATGACATAGTGCAAGG-3'

NM_024551

Glut4

olg404, 5'-ACTGGACGAGCAACTTCATC-3' olg405, 5'-GAGGACCGCAAATAGAAGGAA-3'

NM_001042


Primer sequences were obtained using Primer Express 3.0 software.

Most primer pairs span two exons, except the intronless 18S rRNA. Primers were tested by dissociation curve analysis to assure only single amplicon.

Fang et al. Arthritis Research & Therapy 2012 14:R229   doi:10.1186/ar4070

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