Table 1 |
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|
Primers used for real-time qPCR. |
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|
Gene |
Primer sequences |
Accession number |
|
|
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|
18S rRNA |
olg1, 5'-CCCCATGAACGAGGGAATT-3' olg2, 5'-GGGACTTAATCAACGCAAGCTT-3' |
|
|
GAPDH |
olg3, 5'-CATGAGAAGTATGACAACAGCCT-3' olg4, 5'-AGTCCTTCCACGATACCAAAGT-3' |
|
|
ACTA2 (a-SMA) |
olg20, 5'-CAGGGCTGTTTTCCCATCCAT-3' olg21, 5'-GCCATGTTCTATCGGGTACTTC-3' |
|
|
COL1A1 |
olg149, 5'-GCTGGTGTGATGGGATTC-3' olg150, 5'-GGGAACACCTCGCTCT-3' |
|
|
AdipoR1 |
olg396, 5'-TGACTGGCTAAAGGACAACG-3' olg397, 5'-AAAAGAGAAACAGCACGAAACC-3' |
|
|
AdipoR2 |
olg400, 5'-CAGCCATTATAGTCTCCCAGTG-3' olg401, 5'-CCGAGATGACATAGTGCAAGG-3' |
|
|
Glut4 |
olg404, 5'-ACTGGACGAGCAACTTCATC-3' olg405, 5'-GAGGACCGCAAATAGAAGGAA-3' |
|
|
|
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|
Primer sequences were obtained using Primer Express 3.0 software. Most primer pairs span two exons, except the intronless 18S rRNA. Primers were tested by dissociation curve analysis to assure only single amplicon. |
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|
Fang et al. Arthritis Research & Therapy 2012 14:R229 doi:10.1186/ar4070 |
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