Effects of glucocorticoids or TNFα on Wnt-related gene expression. (A and B) Effect of glucocorticoid treatment (100 nM dexamethasone) or TNFα (10 ng/ml) on expression of Wnt-related genes (n = 96) was examined using a targeted microarray. Genes with the greatest fold change are shown (the effect of TNFα on DKK1 and FRZB expression was not significant). A full list of the genes included in the array and their full gene titles is given in Additional file 1, Table S1. (C) Real-time PCR validation of changes in mRNA expression for FRZB and WNT2 in response to dexamethasone (DEX) or TNFα. *P < 0.05, **P < 0.01 relative to control. Results shown are the combined results of three separate FLS lines.
Hardy et al. Arthritis Research & Therapy 2012 14:R226 doi:10.1186/ar4065