Quantification and characterization of 4-hydroxynonenal (HNE)/protein adducts by enzyme-linked immunosorbent assay (ELISA) and Western blotting. (A) HNE/protein adducts were measured by ELISA in serum and left ventricular (LV) tissues from control and adjuvant-induced arthritis (AIA) rats (n = 6). (B) HNE/protein adducts were characterized by Western blotting in total proteins of LV tissues from control and AIA rats. Values are the mean ± SEM of three experiments. *P < 0.05; **P < 0.01 (CTL versus AIA), #P < 0.05 (AIA versus AIA+Ram) by one-way ANOVA. CTL, control; Ram, ramipril.
Shi et al. Arthritis Research & Therapy 2012 14:R223 doi:10.1186/ar4062