Figure 3.

Human dendritic cells (DCs) are the principal cells implicated in response to smoking. Double immunofluorescence staining of synovial tissue for (A) aryl hydrocarbon receptor (AHR) protein (green) in CMRF44⁺ (red, top panels) or CMRF56⁺ DCs (red, bottom panels). (B) Enlarged images show cytoplasmic and nuclear localization of AHR (green) staining in CMRF44⁺ (red, top panels) or CMRF56⁺ DCs (red, bottom panels) as indicated. (C) CYP1A1 protein (green) in CMRF44⁺ (red, top panels) or CMRF56⁺ DCs (red, bottom panels). (D) AHR and (E), CYP1A1 protein (green) in CD3⁺ T cells (red). In all images, nuclei are counterstained with Hoechst (blue). Scale bar, 20 μm.