Figure 1.

IGFBP-3 and HMGA1 mRNA expression in chondrocytic cell lines. (a) A comparison of IGFBP-3 and HMGA1 mRNA abundance is shown in T/C-28a4 (black columns) and C-28/I2 (gray columns) cells following qRT-PCR of total RNA from three separate experiments. *P = 0.0016; **P = 0.0005. (b) IGFBP-3 and HMGA1 mRNA as measured by qRT-PCR from cultured C-28/I2 cells pretreated with small interfering RNA directed against HMGA1 (gray columns) or untreated (black columns). *P = 0.0046; **P = 0.0004. (c) qRT-PCR of IGFBP-3 and HMGA1 mRNA in T/C-28a4 cells transfected with a pcDNA3/HA-HMGA1 expression vector (gray columns) or untransfected (black columns). *P = 0.0039; **P = 0.0031. Each column indicates the mean value; the error bars indicate standard deviation. HMGA1, high-mobility group A1; IGFBP-3, insulin-like growth factor binding protein 3; qRT-PCR, quantitative real-time reverse transcription-polymerase chain reaction.