Table 2

Extracellular proteins modulated by chondroitin sulfate treatment in IL-1β-stimulated chondrocytes

Accession number^a

Name

Symbol

Ratio^b

P value

Error factor^c

[P00751]

Complement factor B

CFAB

0.5566

0.0064

1.397

[Q08431]

Lactadherin

MFGM

0.597

0.0151

1.3855

[Q7Z7G0]

Target of Nesh-SH3

TARSH

0.7017

0.006

1.2209

[P09871]

Complement C1s subcomponent

C1S

0.7085

0.0007

1.1398

[P01024]

Complement C3

CO3

0.7241

0.0023

1.1999

[P02751]

Fibronectin

FINC

0.7321

0.0006

1.1891

[P03956]

Interstitial collagenase

MMP1

0.7113

0

1.0977

[P00736]

Complement C1r subcomponent

C1R

0.7734

0.0071

1.1755

[P08253]

72 kDa type IV collagenase

MMP2

0.7759

0.0001

1.0999

[P36222]

Chitinase-3-like protein 1

CH3L1

0.8088

0.0216

1.4096

[P10909]

Clusterin

CLUS

0.8224

0.05

1.2162

[P08254]

Stromelysin-1

MMP3

0.8256

0.0086

1.1378

[P07093]

Glia-derived nexin

GDN

1.2894

0.0031

1.1664

[O00391]

Sulfhydryl oxidase 1

QSOX1

1.3064

0.0382

1.2709

[Q92954]

Proteoglycan 4

PRG4

1.4393

0.05

1.445

[P98066]

TNF-inducible gene 6 protein

TSG6

1.7773

0.0007

1.3371

[P61769]

Beta-2-microglobulin

B2MG

2.1652

0.0125

1.4553

[P07996]

Thrombospondin-1

TSP1

89.1678

0

>2

Extracellular proteins identified by SILAC and liquid chromatography-mass spectrometry analysis as modulated by CS treatment in IL-1β-stimulated chondrocytes. ^aProtein accession number according to the SwissProt and TrEMBL databases. ^bAverage SILAC ratios (n = 3) that represent the relative protein abundance in CS-treated versus untreated cells, calculated by Protein Pilot 3.0 software (ABSciex). P ≤ 0.05 was accepted. ^cError factor of the quantification (measure of the error in the average quantification ratio as calculated by Protein Pilot 3.0 software).

Calamia et al. Arthritis Research & Therapy 2012 14:R202   doi:10.1186/ar4040