Table 1

Citrullinated peptides in the ACPA microarray

Peptide

Protein

Amino acids

Amino acid sequence

Reference

CEP-1/Eno5-21

α-Enolase

5-21

CKIHA(cit)EIFDS(cit)GNPTVEC (cyclic)

[16]

Vim60-75

Vimentin

60-75

VYAT(cit)SSAV(cit)L(cit)SSVP

[37]

Vim2-17

Vimentin

2-17

ST(cit)SVSSSSY(cit)(cit)MFGG

[33]

CCP-1/Fil307-324

Filaggrin

307-324

SHQEST(cit)GRSRGRSGRSGS (cyclic)

[3,32]

Fibβ36-52

Fibrinogen β-chain

36-52

NEEGFFSA(cit)GHRPLDKK

[37]

Fibβ563-583

Fibrinogen β-chain

563-583

HHPGIAEFPS(cit)GKSSSYSKQF

[13]

Fibβ580-600

Fibrinogen β-chain

580-600

SKQFTSSTSYN(cit)GDSTFESKS

^c

Fibβ62-81a^a

Fibrinogen β-chain

62-81

APPPISGGGY(cit)ARPAKAAAT

[13]

Fibβ62-81b^b

Fibrinogen β-chain

62-81

APPPISGGGYRA(cit)PAKAAAT

[13]

Fibβ60-74

Fibrinogen β-chain

60-74

(cit)PAPPPISGGGY(cit)A(cit)

[12,31]

Fibα621-635

Fibrinogen α-chain

621-635

(cit)GHAKS(cit)PV(cit)GIHTS

[12,31]

citC1/CII359-369

Collagen type II

359-369

(GPO)5-GA(cit)GLTG(cit)PGDA(GPO)2-GKKYG

[20,28]

The arginine-containing control peptides have identical sequences, except that they contain arginine residues instead of citrulline (Cit, citrulline). Peptides are designated by their abbreviated protein names (Eno, α-enolase; Vim, vimentin; Fib, fibrinogen; CII, type II collagen) followed by the positions of the first and last amino acid. For three peptides, earlier given specific names (CEP-1, CCP-1, citC1), these names are given in parallel. ^aCitrullinated at position 72. Uses the same control peptide containing two arginines as for Fibβ62-81b. ^bCitrullinated at position 74. Uses the same control peptide containing two arginines as for Fibβ62-81a. ^cThe citrullinated epitope in this peptide has been found to be citrullinated in RA synovial tissue (Per-Johan Jakobsson, personal communication).

Hansson et al. Arthritis Research & Therapy 2012 14:R201   doi:10.1186/ar4039